Sex-dependent loss of bisected bovine morulae after culture and freezing
نویسندگان
چکیده
منابع مشابه
Sex determination in bisected bovine embryos and conception rate after the transfer of female demi-embryos
The aim of this study was to evaluate the efficiency of sex determination after microsurgical splitting of D7 (Day 7) bovine embryos and to test the conception rate after subsequent transfer of female demi-embryos. High-quality morulae (n = 100) and early blastocysts (n = 123) obtained from superovulated donors were microsurgically bisected and blastomeres biopsied from one half of bisected emb...
متن کامل“Short Paper” Application of Gamma Irradiation to Increase the Motility and Viability of Bovine Sperm after Freezing and Thawing
This study was conducted to determine the suitable dose of gamma rays for increasing sperm motility and viability after thawing and to assess its effects on malondialdehyde concentration and the rate of sperm DNA strand breaks. Sperm samples in liquid nitrogen were irradiated at doses of zero, 0.1, 0.3, 0.5, 0.7 and 0.9 Gy. The sperm quality and quantity parameters were evaluated before and aft...
متن کاملProduction and development of calves from sexed-bisected bovine embryos.
Sixty-five chromosomal preparations from bisected bovine embryos were examined and 43 embryos (66.2%) with metaphase plates were observed; however, only 25 embryos (38.5%) were sexed. Fifteen of the bisected embryos sexed by chromosomal analysis were transferred to each of the 15 recipients, and 5 recipients became pregnant. Female calves were born on December 28, 1985, August 2, 1986 and July ...
متن کاملI-13: Blastocyst Culture and Freezing
It should now be beyond doubt that blastocyst culture results in better pregnancy rate (PR). This calls for fewer embryos to be transferred, and indeed blastocyst culture is of most value when the intention is to put back one embryo at a time. The PR with one top-grade blastocyst is 45-65% in different studies. If two top-grade blastocysts are transferred, the PR usually does not differ, but th...
متن کاملDifferent chromatin and energy/redox responses of mouse morulae and blastocysts to slow freezing and vitrification
BACKGROUND The ability to cryopreserve mammalian embryos has become an integral part of assisted reproduction, both in human and veterinary medicine. Despite differences in the size and physiological characteristics of embryos from various species, the embryos have been frozen by either of two procedures: slow freezing or vitrification. The aim of our study was to compare the effect of slow fre...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1992
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0960453